How To Inoculate Substrate Jars


How do you inject a substrate jar?

Carefully remove the cap over the syringe needle and slide the needle into one of the holes in the jar lid. Shove it all the way in, so that the needle goes into the cake itself. Gently squeeze out about 1.0-1.5 cc of spore solution into each jar, splitting up the amount if you inject through more than one hole. via

Can I inoculate substrate?

Inoculation of bulk substrates

Bulk substrates are rarely directly inoculated with spores or tissue culture. They are almost always inoculated with a spawn substrate in a process commonly referred to as "spawning". via

How do you inoculate substrate with liquid culture?

When you are ready to inoculate your substrate, transfer the culture to agar, or share some with your friends, tilt the jar so that the liquid reaches the injection port and, with a sterile syringe and needle, suck the culture into the syringe until it's full. via

How do you inoculate substrate with spores?

A syringe contains 20 ml of spore solution, enough to inoculate 6-7 liters of substrate. Just drop a few milliliter in every corner of the mushroom substrate and you are ready to go. After a few days white patches of mycelium will start growing on the places were the spores were dropped. via

How long do inoculate jars last?

Depending on the species of mushrooms, it can take about 1-3 weeks before the jar is fully colonized. At this time you can either add it to a bulk substrate or do a grain to grain transfer to make even more spawn! via

How long does it take for jars to inoculate?

With brf jars usually about 3 to 5 days. WBS seems to take a bit more time to germinate when knocked up with spores about a week to two weeks. they can take 3 weeks to start showing growth and can take up to 2 month to fully colonize . via

Can you inoculate bulk substrate?

Bulk substrates cannot be inoculated using liquid culture or spore solution, and so one of the first steps in a bulk grow is creating grain spawn in order to inoculate the bulk substrate. via

How much gypsum do I add to my substrate?

Gypsum should be mixed at a rate of 2-5% of the dry weight of the substrate. The standard is to add a tablespoon of gypsum for every five half-pint jars. Across all of home mycology, every technique basically calls for a tablespoon or a "handful" of gypsum. It's just a nutrient additive that helps fruiting. via

Can you inoculate sawdust with liquid culture?

With some experience you can also use pure spawn liquid culture to inoculate — this will lead to a faster colonization of the sawdust. via

How many cc's are inoculate?

This will vary depending on if you are using spores or a live culture, and also how potent your syringes are. But generally 1-3 ccs of solution is sufficient to inoculate each 1 pound bags. Use 2-6 ccs per 2 pound bag. via

How do you make liquid culture jars?

Fill the Jar with 400ml of warm water (distilled water preferably). Add 4 tsp of honey to the water (Approximately 16ml). Stir the mixture until the honey dissolves into the water. Place micro-pore tape and foil over the lid covering the hole and screw on lid. via

How do I know when mycelium is fully colonized? (video)

What is the best substrate for Psilocybe Cubensis?

The most suitable substrate for P. Cubensis is said to be rye grain. If grown inside a mason jar – a popular and effective method of cultivation – mycelium will permeate the grain within ten to fifteen days. via

Can you inoculate agar with spore syringe?

Open the petri dish and import the spores to the agar, drawing a „S“ over the media. If using a spore syringe, 1-2 ml solution per petri dish will suffice. It is advisable always to inoculate several petri dishes with a spore print since they thrive and prosper varyingly. via

What is the best mushroom substrate?

The Best Mushroom Substrate

  • Grain – indoor bags of enriched sawdust and pasteurized straw.
  • Sawdust – wood-based substrates like logs, enriched sawdust, wood chips, cardboard, and outdoor straw beds.
  • Dowel/Plug – logs and wood chips.
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